Gene Expression Profiles in Rainbow Trout, Onchorynchus mykiss, Exposed to a Simple Chemical Mixture
Identifieur interne : 000290 ( Main/Exploration ); précédent : 000289; suivant : 000291Gene Expression Profiles in Rainbow Trout, Onchorynchus mykiss, Exposed to a Simple Chemical Mixture
Auteurs : Sharon E. Hook [États-Unis] ; Ann D. Skillman [États-Unis] ; Banu Gopalan [États-Unis] ; Jack A. Small [États-Unis] ; Irvin R. Schultz [États-Unis]Source :
- Toxicological Sciences [ 1096-6080 ] ; 2007-12-15.
Abstract
Among proposed uses for microarrays in environmental toxiciology is the identification of key contributors to toxicity within a mixture. However, it remains uncertain whether the transcriptomic profiles resulting from exposure to a mixture have patterns of altered gene expression that contain identifiable contributions from each toxicant component. We exposed isogenic rainbow trout Onchorynchus mykiss, to sublethal levels of ethynylestradiol, 2,2,4,4-tetrabromodiphenyl ether, and chromium VI or to a mixture of all three toxicants Fluorescently labeled complementary DNA (cDNA) were generated and hybridized against a commercially available Salmonid array spotted with 16,000 cDNAs. Data were analyzed using analysis of variance (p < 0.05) with a BenjamaniHochberg multiple test correction (Genespring [Agilent] software package) to identify up and downregulated genes. Gene clustering patterns that can be used as expression signatures were determined using hierarchical cluster analysis. The gene ontology terms associated with significantly altered genes were also used to identify functional groups that were associated with toxicant exposure. Cross-ontological analytics approach was used to assign functional annotations to genes with unknown function. Our analysis indicates that transcriptomic profiles resulting from the mixture exposure resemble those of the individual contaminant exposures, but are not a simple additive list. However, patterns of altered genes representative of each component of the mixture are clearly discernible, and the functional classes of genes altered represent the individual components of the mixture. These findings indicate that the use of microarrays to identify transcriptomic profiles may aid in the identification of key stressors within a chemical mixture, ultimately improving environmental assessment.
Url:
DOI: 10.1093/toxsci/kfm293
Affiliations:
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However, it remains uncertain whether the transcriptomic profiles resulting from exposure to a mixture have patterns of altered gene expression that contain identifiable contributions from each toxicant component. We exposed isogenic rainbow trout Onchorynchus mykiss, to sublethal levels of ethynylestradiol, 2,2,4,4-tetrabromodiphenyl ether, and chromium VI or to a mixture of all three toxicants Fluorescently labeled complementary DNA (cDNA) were generated and hybridized against a commercially available Salmonid array spotted with 16,000 cDNAs. Data were analyzed using analysis of variance (p < 0.05) with a BenjamaniHochberg multiple test correction (Genespring [Agilent] software package) to identify up and downregulated genes. Gene clustering patterns that can be used as expression signatures were determined using hierarchical cluster analysis. The gene ontology terms associated with significantly altered genes were also used to identify functional groups that were associated with toxicant exposure. Cross-ontological analytics approach was used to assign functional annotations to genes with unknown function. Our analysis indicates that transcriptomic profiles resulting from the mixture exposure resemble those of the individual contaminant exposures, but are not a simple additive list. However, patterns of altered genes representative of each component of the mixture are clearly discernible, and the functional classes of genes altered represent the individual components of the mixture. These findings indicate that the use of microarrays to identify transcriptomic profiles may aid in the identification of key stressors within a chemical mixture, ultimately improving environmental assessment.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Hook, Sharon E" sort="Hook, Sharon E" uniqKey="Hook S" first="Sharon E." last="Hook">Sharon E. Hook</name></region><name sortKey="Gopalan, Banu" sort="Gopalan, Banu" uniqKey="Gopalan B" first="Banu" last="Gopalan">Banu Gopalan</name><name sortKey="Schultz, Irvin R" sort="Schultz, Irvin R" uniqKey="Schultz I" first="Irvin R." last="Schultz">Irvin R. Schultz</name><name sortKey="Skillman, Ann D" sort="Skillman, Ann D" uniqKey="Skillman A" first="Ann D." last="Skillman">Ann D. Skillman</name><name sortKey="Small, Jack A" sort="Small, Jack A" uniqKey="Small J" first="Jack A." last="Small">Jack A. Small</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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